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The road has been closed off to traffic, causing huge congestion.
The Hurriyet newspaper quoted Marmaris mayor Ali Acar as indicating an "error by the driver", without giving further details.
Other media outlets reported that the bus left from the western city of Izmir carrying only women and children who were on a trip for Mothers' Day, celebrated in Turkey on Sunday.
Marmaris is one of the country's main resorts on the Mediterranean, and a popular weekend destination for many Turks as temperatures climb.