Israel deported another 130 activists from the Global Sumud Flotilla to Jordan on Tuesday, a week after it illegally intercepted the vessels off the coast of Gaza.
Local media broadcast footage of buses of activists travelling across the King Hussein Bridge crossing into Jordan from the occupied West Bank.
The individuals included nationals of 22 countries, including Algeria, Turkey, Tunisia, Libya, Oman, Kuwait, the US and the UK, Jordan's foreign ministry said.
Among them was Nelson Mandela's grandson, Mandla, and Jewish-American activist David Adler.
Israel was condemned worldwide after its forces seized more than 40 vessels in the flotilla in international waters and detained more than 450 crew members.
The Sumud flotilla had been attempting to deliver food, medicine and humanitarian aid to Gaza, breaking Israel's siege on the enclave, which has led to the starvation deaths of hundreds of people.
Israel has been accused of mistreating the activists and holding them in harsh conditions.
Israeli legal rights group Adalah said they were prevented from speaking to lawyers and denied access to water and medicine.
Extremist national security minister Itamar Ben-Gvir said he was "proud" of how they were treated.
The Office of the Public Prosecutor in Rome on Tuesday said it was investigating complaints filed by Italian activists over their treatment by Israeli authorities.
Six people remain in Israeli detention, two Moroccan citizens, two Norwegians, a Spanish woman and a US citizen, according to Adalah.
It expects Israel to deport them from the country in the coming days, the organisation told The New Arab.
Israel has been deporting the flotilla participants over the past several days. Some 137 people arrived in Turkey on Monday. Swedish climate activist Greta Thunberg, who was reportedly beaten and forced to hold up an Israeli flag, was deported earlier this week.
Participants in the flotilla included humanitarian workers, lawmakers, journalists and lawyers.
The Global Sumud Flotilla was the third attempt to break the siege this year, after the Madleen and Handala boats were intercepted by Israeli forces.